Gyrolab Podcasts

The Gyrolab^® Spin | Episode 14

Rob and John Talk Spinning Into Assay Development
Rob Durham, Sr. Sales Manager West, and John Chappell, Sr Global Scientific Support Manager, talk about how advanced assay development on the spinning microfluidic CD is fast and easy with these informative tips on assay development. Less common assay formats are discussed including  2-step assay formats and competition assays. Options for assay optimization include CD choice, reagents, buffer, matrices, and the required dynamic range. Make the most of the Gyrolab flexible platform with these great tips.

The Gyrolab^® Spin | Episode 13

Rob and John Talk Assay Troubleshooting and Optimization Part II

Rob Durham, Sr. Sales Manager West, and John Chappell, Sr Global Scientific Support Manager, talk about approaching Gyrolab assay troubleshooting methodically using a process described in a recent Gyrolab User Meeting workshop. Learn from specific case studies on solving problems of low or no signal, optimizing precision, diagnosing negative or no signal data points, correctly handling samples and reagents, and wash station maintenance to optimize immunoassays on Gyrolab platform. 

The Gyrolab^® Spin | Episode 12

Frida Löthberg and Joris Venet Talk Strategies for Measuring Oligo Therapeutics

Rob Durham, Sr. Sales Manager West, and John Chappell, Sr Global Scientific Support Manager, talk about oligo therapeutic assays on the Gyrolab platform with two guest speakers: Frida Löthberg, Application Scientist, and Joris Venet, Field Application Specialist. Frida and Joris discuss strategies for measuring oligonucleotides with Gyrolab assay formats including dual hybridization and nuclease-dependent cutting approaches with micrococcal and S1 nucleases. The dual hybridization assay gave the best sensitivity and specificity, potentially due to the Gyrolab automation technology.

The Gyrolab^® Spin | Episode 11

Rob Durham and John Chappell Talk 20 Yr Anniversary and Global GLS Recap

Rob Durham and John Chappell look back on 20 years of their involvement with Gyrolab technology as customers and as Gyrolab Team members. Rob and John give personal examples of how the Gyrolab Seminar user meetings (GLS) act as the source of many suggestions for platform improvements and application expansions. They go on to recap the hot topics from the GLS meetings around the globe this year held in China, Japan, Europe, and the US. Many emerging topics and assays were presented by Gyrolab users including free target assays, ADC PK assays, microsampling sample collection and Gyrolab drug quantification, cell and gene therapy bioprocess experience, and anti-interferon antibody analysis.

The Gyrolab^® Spin | Episode 10

Rob Durham and John Chappell Talk Talk Generic ADA Assays and Kits on Gyrolab

Rob Durham and John Chappell talk about generic ADA assays on the Gyrolab platform detecting intact immune complexes. Hear about survey results describing market feedback on the utility of generic ADA assays. In addition, they review the assay performance of a new Gyrolab Generic Cyno ADA Kit and the advantages of utilizing the kit for ADA screening in preclinical studies or candidate selection. 

The Gyrolab^® Spin | Episode 9

Rob Durham and John Chappell Talk Affinity – A Practical Guide to Affinity Assay Setup

Rob Durham and John Chappell talk about practical considerations for setting up affinity methods on the Gyrolab platform. Utilizing the Gyrolab system for affinity measurement is very useful for determining binding affinities of analytes such as IgE and omalizumab, or for characterizing reagents. The Affinity Module Software is straightforward and easy to use for bioanalytical scientists of any background, and Rob and John walk through method setup step by step.

The Gyrolab^® Spin | Episode 8

Rob Durham and John Chappell Talk GxP Compliance and Gyrolab Platform

Rob Durham and John Chappell talk about Gyrolab suitability for use in regulated environments during biotherapeutic development. Rob and John discuss software features for 21 CFR Part 11 compliance, validation processes, and ISO 9001 certification. Gyrolab platform has been utilized in regulated environments for over 20 years, learn how to accomplish compliance.

The Gyrolab^® Spin | Episode 7

Rob Durham and John Chappell Talk Gene Therapy Bioprocess Analytics With Gyrolab

Rob Durham, Sr. Sales Manager West, and John Chappell, Sr Global Scientific Support Manager, talk innovative solutions to speed up viral vector characterization in gene therapy development. Rob and John describe disruptive and high-precision approaches to lentiviral and AAV vector titer and bioprocess impurity analysis for bioprocess and QC environments.

The Gyrolab^® Spin | Episode 6

Rob Durham and John Chappell Talk Free vs Total Measurement Using Gyrolab Platform

Rob Durham and John Chappell talk about challenges measuring free versus total therapeutic and equilibrium shifts with conventional plate-based immunoassays. Rob and John discuss how the Gyrolab platform is uniquely designed to measure free analyte, minimizing equilibrium shifts with examples of Avastin, Lucentis, and IgE assays.

The Gyrolab^® Spin | Episode 5 

Rob and John Talk In-Solution Affinity and Kinetics Measurements

Rob Durham and John Chappell talk about in-solution affinity determination and kinetics measurements on the Gyrolab platform. Rob and John discuss straightforward approaches to setting up affinity experiments with examples of TNF-alpha antagonists and their TNF-alpha ligands. The benefits of measuring high-affinity interactions using un-modified interactants in solution are discussed.

The Gyrolab^® Spin | Episode 4

Rob Durham and John Chappell Talk Best Practices for Gyrolab Assay Troubleshooting

Rob Durham, Sr. Sales Manager West, and John Chappell, Sr Global Scientific Support Manager, talk about tips and tricks for assay troubleshooting on the Gyrolab platform. Rob and John cover advanced topics including identifying common causes of poor assay performance, testing and minimizing carry over, and discriminating between instrument and assay issues. Listening to this podcast will tune up your assay development skills!

The Gyrolab^® Spin | Episode 3

Rob Durham and John Chappell Talk Mastering ADA Assays on the Gyrolab Platform

Rob Durham and John Chappell present an informative workshop on understanding and measuring immunogenicity using the Gyrolab immunoassay platform. Rob and John cover topics including defining immunogenicity, measuring anti-drug antibodies (ADAs), cut point analysis, and automated ADA assays using the Gyrolab Mixing CD 96.